102年 - 102 國立嘉義大學_碩士班(甲組)招生考試試題_應用化學系：分析化學#147063

1. Please give a detail procedure, including standardizing with an acid primary standard, for preparation 0.1 N NaOH about 1 L. (4 分)

(A) Please calculate to find the pH values of the two equivalent points. (8 分)

(B) Which of the following indicators is suitable for detecting equivalent point two? And give your reason. (4 分)

3. (A)Please calculate the ionic strength of a solution containing 0.01 M K₂SO₄ and   0.01 M   NaCl.
(5 分)

(B) A solution of 1 liter contains Fe³⁺,SCN^-,and Fe(SCN)²⁺ and reacts following the equation shown as  Fe³⁺(pale yellow) + SCN^-(colorless) ↹Fe(SCN)²⁺(red) to an equilibrium state of red color. If we add 0.01 mole of K₂SO₄ to the present solution, the color will change to deep red or to pale yellow? Please explain why adding inert salt will change the color?(5 分)

4. What are the differences between standard addition and internal standards in quantitative analysis? (5分)

5. Matrix assisted laser desorption ionization and electrospray ionization are two of the popular ionization methods used for mass spectrometry analysis. Please give the principles of the two methods. (5 分)

6. What are the differences between particles packed column, wall coated open tubular column, and capillary electophoresis? Please explain by the relation of van Deemter equation H (plate height) ≈ A (multiple path) + B/u (longitudinal diffusion) + Cu (equilibration time). (5 分)

(A) ion-pair chromatography (3 分)

(B) photodiode array detector (3 分)

(C) inductively coupled plasmas (3 分)