複選題

25. Which of the following is/are category for the gene product for an Oncogene?
(A) Glycolytic enzyme.
(B) Transcription factor.
(C) Housekeeping gene.
(D) Nonreceptor protein kinase.
(E) Growth factor receptor.

複選題26. A mutant bacterium is isolated. This bacterial strain has the characteristic of very slow, "sickly" growth. You isolate mRNA from this bacteria to use in an in vitro translation system and note that even in vitro it is difficult to use this message and obtain protein. Of the following, which is/are likely explanation for these results? (A) There may be a mutation in the ribosomal rRNA recognizing the Shine-Delgarno sequence of the message. (B) In this mutant, the ribosomal subunits may not associate well enough for effective translation. (C) This mutant may have altered tRNA molecules, such that the codon-anticodon interaction during translation is affected. (D) These bacteria may not manufacture enough translation factors for effective translation. (E) There may be a mutation in the Shine-Delgarno sequence of the DNA, resulting in a RNA that poorly binds the ribosome.

27. You are working with a gene associated with an essential pathway that is necessary at two time points in an organism's lifetime. With regard to gene regulation, you would expect the gene to be (A) associated with response to distinct events. (B) not constitutive. (C) "activated" by particular signals (induced). (D) "turned off" by particular signals (repressed). (E) None of the above.

28. In a histone protein, what modification(s) to lysine mark the nucleosome as a transcription target? (A) alkylation (B) acetylation (C) phosphorylation (D) methylation (E) ubiquitylation

29. microRNAs play a key role in which of the following? (A) repressed translation of the mRNA. (B) degradation of viral RNA (C) rapid translation of the mRNA. (D) degradation of the mRNA (E) repeated translation as the miRNA prevents destruction of the mRNA

30. Which of the following statements is/are TRUE? (A) Restriction endonucleases can cut DNA and RNA. (B) It is common to use ddNTPs (dideoxyribonucleoside triphosphates) in DNA sequencing. (C) Enzymes that seal nicks in DNA are called ligases. (D) In recombinant DNA technology, a YAC is an enzyme isolated from a large South American four-legged mammal. (E) The "c" in cDNA stands for Circular.

31. Which property of DNA is crucial for the conservation of genetic information? (A) antiparallelism (B) semiconservative replication (C) the ability to form a circular DNA (D) base-pair complementarity (E) The amount of A is the same as the amount of C

32. The ability of DNA to denature is important for which process? (A) DNA synthesis (B) nucleic acid hybridization experiments (C) RNA synthesis (D) PCR reaction (E) DNA separation by agarose gel electrophoresis

33. Which of the following is required for both DNA replication and RNA transcription? (A) DNA (B) primers (C) RNA polymerase (D) phosphodiesterase (E) topoisomerase

34. Which of the following mobile elements is a retrotransposon? (A) yeast Ty element (B) bacterial IS sequence (C) Drosophila P element (D) maize activator (Ac) element (E) RNA genome of influenza H7N9 virus

35. Which of the following is correct for the eukaryotic chromosomes? (A) The ends of eukaryotic chromosomes are called telomeres, and contain special repeat sequences, called telomeric sequences. (B) Telomere terminal transferase containing the reverse transcriptional activity is important to maintain the chromosomal ends in eukaryotes. (C) The centromeric sequences are important for proper chromosome segregation during replication. (D) Kinetochores assemble at the centrosome. (E) Telomerase activity declines with age and this could explain why cells lose their ability to divide after many replications.

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