13.Which promoter elements of the following RNA polymerase(s) possess TATA box?
(A) RNA polymerase I, II and III
(B) RNA Polymerase II and III
(C) RNA polymerase I
(D) RNA polymerase II
(E) RNA polymerase III

14. Which of the following statements is NOT CORRECT for DNA topoisomerases? (A) DNA topoisomerases can cleave DNA backbone at phosphodiester bond (B) Only participate enzymatically in overwinding, knotting and catenation of DNA (C) Relive the torsion stresses associated with DNA replication and transcription elongation (D) Both type I and II topoisomerase change the linking number of DNA (E) Human topoisomerase I is the antigen recognized by Anti-Scl-70 in scleroderma

15.Typically, protein-protein can be detected by yeast two-hybrid and co-immunoprecipitation assays as well as which of the following methods? (A) RNAi screening technology (B) Yeast one-hybrid (C) Gel mobility shift assay (D) Farwestern blotting analysis (E) CRISPER-Cas9 technology

16. Which one of the statements about amino acid is NOT TURE? (A) The essential amino acid is due to that they can not be produced from other compounds of human body (B) There are 10 proteinogenic amino acid are called "essential" (C) Amino acids have both the amine and the carboxylic acid groups attached to the first (alpha-) carbon. (D) There are about 500 naturally occurring amino acid (E) Only 20 amino acid appear in the genetic code

17. Which one of the statements about heterochromatin is NOT CORRECT? (A) It is often tightly packed form of DNA (B) There are facultative heterochromatin and constitutive heterochromatin and both play a role in the expression of genes (C) Heterchromatin has been associated with the di- and tri-methylation of H3K19 in certain portions of the genome (D) Euchromatin and heterochromatin could be distinguished cytologically by how intensively they are stained, and typically heterochromatin stains intensely while euchromatin is less intense (E) Heterochromatin consists mainly of genetically inactive satellite sequences

複選題18. Which of the following macromolecules have catalyst function in the biological system? (A) RNA (B) Protein (C) Lipid (D) Carbohydrate. (E) Fatty acid

19. Which of the following is INCORRECT about the inactive state of the Ras protein? (A) Cell proliferation activation (B) Gene mutation leads to cancer development (C) Changes conformation when bound to regulatory molecules (D) GDP bound form (E) The G1 motif of the five G motifs binds to GDP

20. Which of the following types of resistance is not provided by the plasmid for its host? (A) Antibiotics resistance (B) Heat resistance (C) Toxic resistance (D) Phage infection (E) Heavy metals

複選題21. Which of the following chemical bonds are involved in the stabilization of two proteins in one complex? (A) H-bond (B) Ionic bond (C) Van der Waals (D) Salt bridge (E) Peptide bond

22.Allosteric control of O2 binding is present in hemoglobolin but not myoglobin. This is because hemoglobin protein has (A) Quaternary structure (B) Tertiary structure (C) Secondary structure (D) Changes in conformation of subunit after oxygen binding (E) Iron

23.An enzyme is a catalyst for a specific reaction by (A) decreasing ΔG (B) decreasing ΔΗ (C) decreasing ΔG‡(intermediate) (D) decreasing S (E) increasing the reaction rate

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