22. Which of the following factor is involved in termination of mRNA transcription in prokaryotes.
(A) σ factor
(B) p factor
(C) ẞ subunit
(D) a subunit
(E) ATP.

23. The initiation complex of eukaryotic mRNA transcription usually forms at the position relative to the start site (+1) around (A) -200 (B) +100 (C) +30 (D) -30 (E) -1

24.IPTG-induction of ẞ-galactosidase activity is the result of(A) stimulation of Lac repressor function(B) IPTG binding to the lac operon and inducing transcription.(C) IPTG binding to the lacl gene product and inhibiting its activity.(D) inhibition of ẞ-galactosidase degradation.(E) IPTG stimulates enzymatic function of ẞ-galactosidase

複選題25.E. coli does not express ẞ-galactosidase when its growth medium containing both lactose and glucose, because (A) CAP is not activated for DNA binding to promote transcription initiation. (B) Glucose activates the expression of lac repressor. (C) Lac repressor can still bind to the lac operator sequence. (D) Glucose interferes with lactose binding with the lac repressor. (E) Glucose inhibits lac operon

複選題26. Which of the following factors is essential for mRNA maturation in eukaryotes(A) 5'Capping complex(B) polyA addition to 3' tail(C) Splicesome(D) TATA-box binding protein(E) Carboxyl-terminal domain of pol II largest subunit

27. Which of following double-strand DNA sequences has highest melting temperature?(A) gga tta cct agc(B) ggc tta cct agc(C) ggc cta cct agc(D) ggc cca cet agc(E) ggc ccc cct agc

28.In Watson-Crick model of double-helical DNA, how many bases form one turn of helix?(A) 8(B) 9(C) 10(D) 14(E) 16

29.Exonuclease activity is important for DNA polymerase function because(A) It randomly removes the newly incorporated nucleotide.(B) It removes oxidized base.(C) It removes mismatched nucleotide in the exonuclease site for proofreading.(D) It removes primers present in the lagging strand during DNA replication(E) It increases DNA polymerization reaction

30. Which of the following RNA has the highest percentage of modified bases(A) mRNA(B) HnRNA(C) rRNA(D) tRNA(E) Micro-RNA

複選題31. Which of following bonds do not belong to covalent bonds:(A) disulfide bond.(B) hydrogen bond.(C) phosphodiester bond.(D) peptide bond(E) van der Waals

複選題32. Which of following statement(s) for nucleic acid is/are True: (A) C, T are Purines (B) There are two hydrogen bonds between CG pairing (C) During the process of free nucleotides becoming RNA, every single nucleotide added, two phosphate groups are released. (D) The higher the GC content, the lower the Tm value is. (E) RNA at neutral pH buffer (pH=7) has no charge

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