24. There is a preinitiation complex of the eukaryotic ribosome formed before translation. What could be the component within this complex?
(A) 60S
(B) eIF2·ATP+Met-tRNAiMet
(C) eIF1B
(D) 18S
(E) GDP

25. Which of the following statement(s) is correct regarding to the membrane transport?1. Integral membrane protein is likely to contain one or more hydrophobic segments, 20-30 amino acids long.2. Exocytosis involves the cell engulfing matter from the outside environment.3. The voltage-gated potassium channels associated with an action potential provide an example of coupled transport (A) 1 and 3 (B) 2 and 3 (C) 1 only (D) 1 and 2 (E) All of above are correct.

26. You are studying the entry of a molecule into red blood cells. You determine the rate of movement across the membrane under a variety of conditions and carry out the following results: 1. The molecules can move across the membrane in either direction. 2. The molecules always move down their concentration gradient. 3. No energy source is required for the molecules to move across the membrane. 4. As the difference in concentration across the membrane increases, the rate of transport reaches a maximum. The mechanism used to get this molecule across the membrane is most likely: (A) simple diffusion. (B) facilitated diffusion. (C) active transport. (D) pinocytosis. (E) There is not enough information to determine a mechanism.

27. Which of the following statement(s) regarding cytoskeleton is correct?1. made from the repetitive assembly and disassembly of dynamic protein components2. comprising the cytoskeleton are microtubules, intermediate filaments, and actin filaments3. in neurons, intermediate filaments is also called neurofilaments (A) All of above are correct (B) 1 and 3 are correct (C) 2 and 3 are correct (D) 3 only is correct (E) 2 only is correct

28. Which of the following statements regarding stem cells is correct?1. Stem cells can do asymmetric cell division.2. Cord blood stem cells are pluripotent.3. Induced pluripotent stem cells can differentiate into any of the three germ layer cells. (A) 1 and 2 (B) 1 and 3 (C) 2 and 3 (D) None of above (E) All of above

29. Which of the following statements regarding cell-cell and cell-matrix adhesion is incorrect?1. The major families of cell surface adhesion molecules include integrins.2. Vertebrate gap junctions are composed of adherins.3. A tight junction is made up of integrins.4. NCAMs, a group of cell-adhesion proteins belonging to the Ig superfamily, bind to proteoglycans. (A) 1 and 2 (B) 1 and 3 (C) 2 and 3 (D) None of above (E) All of above

30. Which one mainly regulates the replication stress in the S phase? (A) ATM (B) ATR (C) DNA-PKcs (D) Aurora A (E) APC/C

31. Which one can be used in cancer immunotherapy? (A) Anti-PD1 antibody (B) Anti-PDL1-antibody (C) Anti-CTLA4 antibody (D) CAR-T (E) All of these

32. In the large-scale production of a particular human protein in E. coli cells, the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the N-terminus. The purpose of this modification was to (A) facilitate transfer of the cDNA into the E. coli cells. (B) provide a promoter for the transcription of the cDNA in E. coli. (C) facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms. (D) prevent degradation of the expressed protein by E. coli proteases. (E) facilitate translation rate.

33. In RNA interference studies, the double-stranded RNA (A) disrupts the target DNA sequence. (B) results in the destruction of the target mRNA. (C) destroys the target protein. (D) all of the above (E) none of the above

34. In two-dimensional gel electrophoresis, proteins are first resolved by ______ and then by ______ (A) IEF； gel filtration (B) SDS-PAGE； affinity chromatography (C) SDS-PAGE； ion exchange (D) IEF； SDS-PAGE (E) gel filtration； SDS-PAGE

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