27. Which of the following is NOT present in the core RNA polymerase?

(A)β

(B) β'

(C) σ

(D) None of the above.

1. Which of the following is not present in the core RNA polymerase? (A) β (B) β' (C) σ (D) both ẞ and σ

28. Which of the following approaches would you use to loosen the interaction of the histones with DNA in an extract that you have prepared? (A) methylate the DNA (B) methylate the histone (C) acetylate the histones (D) phosphorylate the histones

29. One method used by researchers to demonstrate the existence of nucleosome free DNA regions is ________.(A) RNA Polymerase Run-off experiments. (B) Nuclear Run on experiments. (C) Reporter gene experiments. (D) DNase hypersensitivity experiments.

30. You need to design PCR primers to amplify following gene, what will your PCR primers look like?5'ATCGACCTAGATCGCAGCTCGTAGTCGATGTCA........GCTAGCTGTGCTAGCTGATGCTAAATCGATCGAT-3'(A) Forward: 5'-ATCGACCTAGATCGCAGC-3' Reverse: 5'-GATGCTAAATCGATCGAT-3'(B) Forward: 5'-ATCGACCTAGATCGCAGC-3' Reverse: 5'-ATCGATCGATTTAGCATGA-3'(C) Forward: 5'-GCTGCGATCTAGGTCGAT-3' Reverse: 5'-GATGCTAAATCGATCGAT-3'(D) Forward: 5'-GCTGCGATCTAGGTCGAT-3' Reverse: 5'-ATCGATCGATTTAGCATGA-3

31. Which of the following is NOT true ? (A) The coding sequence (CDS) is the actual region of DNA that is translated to form proteins. (B) An open reading frame (ORF) is the DNA sequence between translation start codon and stop codon. (C) In eukaryotic cell, the ORF may longer than CDS because ORF may contain introns as well. (D) There are three possible ways of translating nucleotide sequence into amino acid sequence according to the genetic code. These are called reading frames.

32. Which of the following is NOT true? (A) Because DNA polymerases polymerize DNA only in the 5'-to-3' direction, synthesis of the lagging strand at a replication fork must occur discontinuously through a backstitching mechanism that produces short DNA fragments. (B) When the replication fork reaches an end of a linear chromosome: there is no place to produce the RNA primer needed to start the last Okazaki fragment at the very tip of a linear DNA molecule. (C) In human, telomeres consist of many tandem repeats of a short sequence that contains a block of GGGTTA, extending for about 10,000 nucleotides. (D) The telomerase synthesizes a new copy of the repeat, using a loop-back protruding single-stranded DNA template from another strand.

33. Which of the following is NOT true about proteasome? (A) Proteasome contains abundant ATP-dependent proteases. (B) Present in many copies dispersed throughout the cytosol and the nucleus. (C) Each proteasome consists of a central hollow cylinder formed from multiple protein subunits. (D) The proteasomes act on proteins that have been specifically marked for destruction by the covalent attachment of multiple copies of selectin.

34. Which of the following assay can be used to detect sequence-specific DNA binding protein?(A) Gel-Mobility Shift Assay(B) DNA Affinity Chromatography(C) Chromatin Immunoprecipitation(D) All of the above

35. Approximately what fraction of the human genome are exons of all genes?(A) 1%(B) 10%(C) 25%(D) 50%

36. 2. BamHI has a restriction site of G↓GATCC while Hpal has a restriction site of GTT ACC. Based upon this, BamHI produces____________ and Hpal produces__________.(A) a 5'overhang; a 3'overhang (B) a 5'overhang; blunt ends (C) a 3'overhang; a 5'overhang (D) a 3'overhang; blunt ends 

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