30. Which sequences are removed in mature mRNA of chicken ovalbumin gene?
(A) intron
(B) exon
(C) polyadenylation sequences
(D) cap sequences

31. What is used to break intron/exon junction by RNA catalyzed splicing of RNA in protozoan Tetrahymena thermophila? (A) guanosine 3'OH (B) adenosine 2'OH in intron (C) small nuclear ribonucleoproteins (snRNPs) (D) endonuclease and ATP

32. 7-methylguanosine is added to the 5' end of almost all eukaryotic mRNAs in 5',5'-triphosphate linkage (A) at transcription initiation (B) at transcription elongation (C) after transcription (D) none of above

33. Which sequence at the 3'-untranslated region is involved in polyadenylation and regulation of mRNA stability of lung cells? (A) AAAAAU (B) AAUAAA (C) UUUUUA (D) UUAUUU

34. Which description is not correct for the flow of genetic information? (A) DNA to RNA by RNA polymerase (B) RNA to DNA by reverse transcriptase (C) RNA to RNA by reverse transcriptase (D) RNA to protein by ribosome-associated translation

35. Rous sarcoma virus genome is indicated below. Which gene is not necessary for the maintenance of the life cycle of this RNA virus? (A) gag (for packing genome) (B) pol (for genome replication) (C) env (for packing virus particle) (D) src (for oncogenesis)

36. Which description   not   correct for telomerase? (A) it is a specialized reverse transcriptase (B) its activity is important for maintenance of telomere or chromosome ends (C) its contains RNA as template for extension of chromosome DNA at end (D) loss of telomerase is cancer-prone

37. Which description is   most   appropriate for Sp1, a transcription enhancer-binding protein in transcription? (A) its binding is position-independent (B) its binding is orientation-independent (C) its binding is position-dependent and orientation-dependent (D) its binding is position-independent and orientation-independent

38. Which description is   most   appropriate for RNA interference (RNAi)? (A) it involves the control of gene expression (B) it down-regulates gene expression through inhibiting translation (C) it down-regulates gene expression through enhancing mRNA degradation (D) all of above

39. If an mRNA was specifically localized at one side of the cell cortex, what would not be the mechanism for this localization? (A) mRNA was only transcribed at one side of the cell (B) at the other side of the cell, mRNA was degraded rapidly (C) an anchoring protein which binds to the 3'UTR of the mRNA was localized at one side of the cell cortex (D) mRNA was directly transported towards this side of cell

40. How do you visualize the localization of protein in cells? (A) RNA in situ hybridization (B) Western Blotting (C) Proteomic analysis (D) Immunofluorescent staining

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