(a) endoplasmic reticulum (ER)

(b) Golgi apparatus?

二、問答題 1. Both bacterial strains A and B possess gene X on their chromosomes. However, only strain A shows a positive reaction with anti-protein X antibody in the Western analysis. What mutation(s) does strain B have? Describe an experiment to identify the possible mutation(s). 

2. At 8:00 a.m. a closed flask of sterile broth is inoculated with 10,000 bacteria. The lag phase lasts 2 hour. At 4:00 p.m. the log phase culture has a population of 1 x 107 bacteria. What is the generation time of this bacterium? Please include your calculation in your answer. 

3. Read the following paragraph and answer the question: Using transgenic plants as a novel and safe system for vaccine production, which could become an attractive alternative in animal health care. The fact that feeding animals with transgenic plants expressing a recombinant antigen caused a systemic immune response demonstrates the viability of using recombinant plants as both an expression and delivery system for oral vaccines. The establishment of plant-based immunization methods for microbial antigens may be an inexpensive alternative to conventional fermentation systems for vaccine production, as plants can be grown inexpensively in large quantities and provide a practical and feasible delivery system. Question: Please name   two   advantages of using transgenic plants for vaccine production. 

(a) What is the full term for "PCR" described above? 

(b) Explain why PCR is an indispensable technique in medical and biological research. 

二、簡答題：1.請說明主管機關在哪些情況下，可依法廢止放射性廢棄物處理設施運轉人員之認可證書?

2.請依放射性物料管理法施行細則第31條規定，至少列舉四項放射性廢棄物貯存設施之異常或緊急事件為何？

三、申論題：1.某設施經營者規劃將放射性廢棄物進行均勻固化處理，且將向主管機關提出固化流程控制計畫，請說明該計畫應載明之事項有哪些?另請說明安定化處理之定義為何?

2.某公司申請使用盛裝容器，請依放射性廢棄物處理貯存及其設施安全管理規則第9條之規定，說明報告應載明那些事項？另外，裝有放射性廢棄物之盛裝容器表面非固著性污染限值為何？

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