2. 問卷調查是我們日常生活中十分常見的資料蒐集工具，舉凡民意調查、顧客滿意度、方案評估…等均可使用。試針對結構式(structured)問卷的設計，給予 5 個發問問題的指引，使變項得到較好的操作化，以避免導致無用甚至誤導資訊的陷阱產生。(請舉出 5 個指引並針對每個指引詳細解釋，每個 10 分，共 50 分)。

II.Short Questions (40%) 1. A nonapeptide was determined to have the following amino acidcomposition: (Lys)2, (Gly)2, (Phe)2, His, Leu, Met. The native peptidewas incubated with 1-fluoro-2, 4-dinitrobenzene (FDNB) and thenhydrolyzed 2,4-dinitrophenylhistidine was identified by HPLC. Whenthe native peptide was exposed to cyanogen bromide (CNBr), anoctapeptide and free glycine were recovered. Incubation of thenative peptide with trypsin gave a pentapeptide, a tripeptide, andfree Lys. 2,4-Dinitrophenyl- histidine was recovered from thepentapeptide, and 2,4-dinitrophenylphenylalanine was recoveredfrom the tripeptide. Digestion with the enzyme pepsin produced adipeptide, a tripeptide, and a tetrapeptide. The tetrapeptide wascomposed of (Lys)2, Phe, and Gly. What is the native sequence?(10%)

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