25. During mRNA splicing, the branch point is recognized by _____ snRNP. (A) U1 (B) U2 (C) U3 (D) U6

26. The catalytic center of a mRNA splicing complex is form by _____ snRNPs. (A) U2AF (B) U2/U5 (C) U2/U4 (D) U2/U6

27. The Tetrahymena 26S rRNA group I intron _____.(A) splices without protein factor (B) is cut by Ul/U5 snRNP (C) is cut by U2/U6 snRNP (D) is spliced as GU-AG intron

28. The poly （Ａ） tail of a mRNA is _____.(A) added during late transcription (B) located right before 3'-UTR (C) synthesized by RNA polymerase II (D) encoded in the being transcripted gene

29. An experiment was designed to obtain nonspecific transcription from both strands of a DNA molecule. Which of the following strategies would be most effective in achieving this? (A) Include the RNA holoenzyme in the reaction. (B) Use the core enzyme of RNA polymerase. (C) Enrich the preparation with sigma subunit. (D) Use intact DNA.

30. Which of the following techniques is suitable for the study of DNA RNA hybrid formation during transcription? (A) DNA:protein crosslinking (B) DNAase I footprinting (C) DNA:RNA crosslinking (D) two-dimensional PAGE

31. Which of the following plasmids could be used to restore inducible regulation of β-galatosidase in this mutant: ? (A)(B)(C)(D)

32. Which of the following would be effective in blocking transcription from the lac operon in the presence of permease? (A) antibody to RNA polymerase (B) antibody to the repressor protein (C) mutation in the operator (D) an antibody to β-galactosidase

33. Which of the following explains the events in late stage T4 infection in bacteria? (A) The host sigma factor specifies transcription of genes. (B) There is a high level of transcription of host genes. (C) Host polymerase holoenzyme directs transcription. (D) The host core enzyme participates in transcription conjunction with phage-encoded specificity factors.

34. Superinfection of lysogenized -infected (E) coli is prevented because (A) the superinfecting phage DNA is rapidly degraded by the host. (B) the superinfecting phage DNA is inhibited by excess repressor binding. (C) the superinfecting phage cannot replicate due to insufficient nucleotide precursors. (D) the superinfecting phage DNA is rapidly degraded by the host and the superinfecting phage DNA is inhibited by excess repressor binding are correct.

35. You have just discovered a new putative repressor in a strain of (E) coli that was sent to you by a colleague. One of the best ways to test for the specificity of this repressor for an operator sequence is to (A) replace the putative DNA-binding site with the binding site from another repressor. (B) change some of the amino acids in the binding site by site-directed mutagenesis. (C) insert an additional segment of amino acids next to the DNA binding site. (D) replace the putative DNA-binding site with the binding site from another repressor and change some of the amino acids in the binding site by site-directed mutagenesis.

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