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115年 - 115 專技高考_大地工程技師(一)：材料力學#140592(44題)

25. Which of the following statement is correct related to general and gene-specific transcription factors in eukaryotes? (A) TATA-box-binding protein (TBP) binds to the minor groove of DNA, bends the consensus TATA DNA, and is required for most members of class I, II and III genes. (B) For RNAP III, TBP is a component of the general transcription factor TFIIIC. (C) The bZIP transcription factors dimerize through a basic domain and bind to target DNA through a leucine zipper. (D) TFIIE and TFIIH are essential for promoter clearance and transcription elongation. (E) The Cys2His2-zinc finger of TFIIIA is composed of an antiparallel beta-sheet followed by an alpha-helix, and the beta-sheet recognizes and binds to specific DNA sequences in the major groove.

24. Which of the following statement is correct in eukaryotic transcription? (A) Yeast Gcn4 controls amino acid metabolism, containing a domain using the basic region to bind to DNA and helix-loop-helix to form a protein dimer. (B) The carboxyl-terminal domain of RNA polymerase is phosphorylated prior to the formation of the initiation complex. (C) By adding of excess acetyltransferase to in vitro transcription assay, it is likely lead to a tighter association of histone with DNA, resulting in reduced transcription. (D) TAF1 of the general transcription factor TFIID is a helicase and a histone acetyltransferase. (E) The high mobility group protein (HMG I(Y)) plays a role in interferon-ẞ (IFN-ẞ) transcription by bending the DNA, causing more activators to bind and interact with each other to enhance transcription.

23. Which of the following is true for eukaryotic Class I and III general transcription factors, except: (A) TFIIIB and TFIIIC are required for all "classical" class III genes. (B) TFIIIC is an assembly factor that helps TFIIIB to bind to a region just upstream of the transcription start site. (C) Human SL1 is an assembly factor that helps the UPE-binding factor of RNA polymerase I to bind to the core promoter element. (D) TFIIIA is a zinc finger protein & is required for transcription of the 5S rRNA genes, but not the tRNA genes. (E) For the class I transcription, human SL1 consists of TATA-binding protein (TBP) and TBP-associated factors (TAFs), which are different from TAFs found in TFIID.

10. Why is a frameshift mutation located at the beginning of a coding region generally more detrimental to protein function than a missense mutation? Briefly explain the reason. (4 pts)

9. You observe that adding DNase to a bacterial culture prevents the bacteria from acquiring a new hereditary trait. Based on this observation, which gene transfer mechanism is occurring (Transformation, Transduction, or Conjugation)? Explain your reasoning. (4 pts)

8. In quantitative PCR (qPCR), why do we focus on the Ct value (Threshold Cycle) rather than the final amount of product at the end of the reaction? (3 pts) Briefly define what the Ct value represents. (3 pts)

全彩圖解英文音標發音課-自然發音+KK音標一次全學會！

講師：台中大雅家教-睿智數學及英文家教，王瑞志老師

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高一下英文課程(十年級)

講師：台中大雅家教-睿智數學及英文家教，王瑞志老師

簡介：高一下單字課，老師講解，偶爾有片語。

高二下英文課程

講師：台中大雅家教-睿智數學及英文家教，王瑞志老師

簡介：高二英文下單字課

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課程：國中國文
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tina測試

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課程：國中國文
章節：章節

6.動態評量(dynamic assessment)是以下列何種理論為基礎？ (A)Freud的心理分析論 (B)Piaget的認知發展論 (C)Bandura的社會學習論 (D)Vygotsky的社會發展認知論

20.考試是學生主要的壓力來源之一，面對即將進入國三的學生，國中基本能力測驗是一場不可避免的重大考 試。某國中校長希望在開學之初找出對壓力反應傾向較高的學生，以便協助因應考試壓力。下列測驗何者最 合適？ (A)情境焦慮量表 (B)特質焦慮量表 (C)貝克憂鬱量表(BDI-II) (D)貝克焦慮量表(BAI)

34.本月因工作量增加，員工有加班，並請了2名臨時工讀生，下列對成本之敘述，何者正確？ (A)員工基本薪水＋加班費屬半變動成本 (B)臨時工讀生屬於半變動成本 (C)臨時工讀生屬於固定成本 (D)員工基本薪水屬變動成本

20.甲公司於×5 年 1 月 1 日有保留盈餘$500,000，流通在外普通股股數為 200,000 股，面額$10。當年度公司發生之交易如下：1.宣告現金股利每股$1；2.宣告並發放 5%的股票股利，目前股票市價為每股$18；3.當年度淨利$460,000。甲公司×5 年 12 月 31 日保留盈餘餘額為多少？ (A) $760,000 (B) $660,000 (C) $580,000 (D) $960,000

5.流式細胞儀操作中，下列敘述何者錯誤？ (A)與 488 nm 雷射光垂直的光徑偵測器無法提供細胞大小的數據 (B)激發的紅色螢光與綠色螢光信號可能會互相干擾  (C)細胞沒有染色則無法看出細胞內的顆粒密度 (D)細胞表面分子可以活染不需固定細胞

7.下列細胞激素何者無法幫助肥大細胞的發育與成熟？ (A)IFN-γ (B)IL-3 (C)IL-4 (D)IL-9