15. Which of the following enzymes is absolutely required for the construction of cDNA library?
(A) reverse transcriptase
(B) polynucleotide kinase
(C) terminal transferase
(D) DNA polymerase I
(E) DNA topoisomerase

16. Which of the following tRNA anticodon is complementary to the mRNA codon 5'UCG3'? (A) 5'AGC3' (B) 5'AGU3' (C) 5'UGA3' (D) 5'UGG3' (E) 5'CGG3'

17. Which one of the following statements in regard to the prokaryotic transcription and translation is incorrect? (A) RNA polymerase transcribes all of mRNA, tRNA, and rRNA. (B) One mRNA encodes one protein (C) Lactose operon is regulated by CAMP (D) Transcription is coupled with translation (E) Transcription does not occur in nucleus

18. Ribosome group A was labeled with and ribosome group B was labeled with . Six hours after addition of both ribosome groups to an in vitro translation system, how many bands of 70S ribosomes are you expected to appear in a density gradient? (A) 1 (B) 2 (C) 3 (D) 4 (E) 5

19. Which one of the following statements in regard to Rho-dependent transcriptional termination in prokaryotes is incorrect? (A) Hexamer of Rho factor binds to upstream G-rich region (B) Rho binds to transcript and pursues RNA polymerase (C) Rho catches up RNA polymerase when RNA hairpin forms and RNA polymerase pauses (D) Rho helicase releases transcript and causes termination (E) Rho can be recycled and binds to new transcript

20. Which of the following splicing needs enzymatic activity of protein for cleavage? (A) Group I self-splicing (B) Group II self-splicing (C) Nuclear rRNA processing (D) Nuclear tRNA intron splicing (E) None of above

21. Which of the following compounds can activate G protein? (A) Norepinephrine (B) Phorbol ester (C) Ouabain (D) Insulin (E) Erythropoietin

22. What is the molecular size of immunoglobin G? (A) 5 kDa (B) 50 kDa (C) 150 kDa (D) 1 nm in diameter of the molecule (E) 5 nm in diameter of molecule

23. Which of the following assays can be used to determine cytokine concentration in the blood? (A) polymerase chain reaction (B) agarose gel electrophoresis (C) polyacrylamide gel electrophoresis (D) enzyme immunoassay (E) isoelectric focusing

24. Which of the following amion acids can undergo a series of reaction to form the particular odor of feces? (A) Glutamate (B) Asparagine (C) Tryptophan (D) Arginine (E) Tyrosine

複選題25. Iodination can occur in which functional group in the proteins? (A) -NH2 group (B) -OH group (C) -COOH group (D) glyco-moiety (E) phenyl group

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