15. Which of the following statements is   True   regarding Cas9?
(A) It was first found in virus
(B) It was first found in parasite
(C) It is a RNA endonuclease
(D) It is a DNA endonuclease

16. Which of the following statements is   True   regarding the stacking gel used in SDS-PAGE? (A) It has a high pH relative to that of the resolving gel (B) It is usually located at the bottom of the resolving gel (C) It has a lower polyacrylamide concentration than that of the resolving gel (D) Its pH value is normally pH8.8.

17. ELISA is the abbreviation of Enzyme-linked immunosorbent assay. Which of the following enzymes is   NOT   routinely used for ELISA? (A) alkaline phosphatase (B) glucose 6-phosphate dehydrogenase (C) Taq DNA polymerase (D) horseradish peroxidase

複選題18. Which of the following statements about Western Blotting is   CORRECT  ? (A) The detection of a particular protein by Western Blotting relies on labelling the protein with a specific dye (B) The detection of a particular protein by Western Blotting relies on labelling the antibody with a specific dye (C) The detection of a particular protein by Western Blotting relies on the very specific interaction between the protein and its antibody (D) The detection of a particular protein by Western Blotting relies on the denaturation of the protein

19. Which reaction in DNA replication is catalysed by DNA ligase? (A) Addition of new nucleotides to the leading strand (B) Addition of new nucleotides to the lagging strand (C) Base pairing of the template and the newly formed DNA strand (D) Formation of a phosphodiester bond between the 3'-OH of one Okazaki fragment and the 5'-phosphate of the next on the lagging strand

20. Which of the following statements regarding splicing of eukaryotic mRNA transcripts is   CORRECT  ? (A) Exons are spliced out and introns are retained in the mature mRNA transcript (B) Small nuclear RNAs are retained in the mature mRNA transcript (C) Several reactions in the splicing process involve hydrolysis of ATP (D) Splicing takes place in the cytosol

21. Which of the following enzymes is   NOT   required during mammalian DNA replication? (A) T4 DNA polymerase (B) DNA ligase (C) DNA helicase (D) Primase

22. All of the followings are involved in translation of protein in a cell EXCEPT (A) rRNA (B) siRNA (C) mRNA (D) tRNA

23. Which of the followings is a DNA molecule? (A)(B)(C)(D)

24. In which phase of the cell cycle does DNA replication occur? (A) GO (B) G1 (C) S (D) M

25. DNA methylation is associated with: (A) CAT box (B) CpG island (C) TATA box (D) enhancer

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