23. Skeletal muscle does not use which of the following nutrient as fuel:

(A) Free fatty acids.

(B) Ketone bodies.

(C) Glucose.

(D) Amino acids.

(E) None of the above.

24. An isoschizomer is a(n)(A) enzyme that cuts DNA from the 3' end.(B) restriction enzyme that has the same sequence specificity as another restriction enzyme from a different organism.(C) DNA sequence that is identical to one in a different organism.(D) DNA sequence from a virus that mimics a sequence in bacteria.(E) None of these is correct.

25. Cartilage and mucous are both slippery because:(A) Short polymers comprise these compounds.(B) The charge repulsion between the many acid groups in these polymers.(C) The sticky nature of sugars.(D) Both charge repulsion of acidic groups and the sticky nature of sugars.(E) All of these.

26. Mr. Bean synthesizes a pentapeptide "DIRTY". Choose the correct answer from the following descriptions (To calculate pl, use the pK2 of the N-terminal amino acid residue as the pKa of the N-terminal amino group of the peptide and pK, of the C-terminal amino acid residue as the pKa of the C-terminal carboxylate group of the peptide):1. The pl value of the peptide is 6.625.2. The pl value of the peptide is 2.925.3. The pl value of the peptide is 6.38.4. When 0.1 mg of the peptide is dissolved in 100 mM Tris (pH 9), it could bind to anion exchange column.5. When 0.1 mg of the peptide is dissolved in 100 mM Tris (pH 9), it could bind to cation exchange column.(A) 1 and 4 are correct.(B) 2 and 4 are correct.(C) 1 and 5 are correct.(D) 3 and 4 are correct.(E) 3 and 5 are correct.

複選題27. Please choose the answer from the following descriptions:1. This figure (right side) tells you & angle is 180 degree.2. Glycogen is more extensively branched than starch.3. Run SDS-page twice is the way to get 2D-PAGE for proteomic analysis.4. 180 g glucose and 180 g sucrose have the same osmotic pressure.5. 1M glucose and 1 M sucrose have the same osmotic pressure.(A) 1,2, and 5 are correct.(B) 1 and 5 are correct.(C) 1, 2, and 4 are correct.(D) 3 and 5 are incorrect.(E) 2 and 5 are correct.

28. Emma wants to analyze the sequence of the following peptide "SMALLCATSNICKASHIRT". She uses several agents to treat the sample. Which of the following description is correct?(A) If she uses trypsin, she could get the peptide "KASHI".(B) If she uses phenylisothiocynate, she could only know that the first residue is "S".(C) If she uses trypsin, she could get the peptide "SMALLCATSNICK".(D) In order to know whether this peptide has disulfide bond, she can treat the sample with or without cyanogen bromide (CNBr) before trypsin digestion and compare the result. If the treatment of cyanogen bromide makes no difference, the result suggests no disulfide bond formation in this peptide.(E) If she uses pepsin, she can get the peptide "ATSNICKASHIRT",

29. Comparing X-ray Crystallography, Circular Dichroism (CD) spectroscopy, Mass spectroscopy and Nuclear Magnetic Resonance (NMR) spectroscopy, choose the incorrect answer:(A) X-ray diffraction data yields electron density map; CD data yields protein secondary structure.(B) Mass spectroscopy could be used in peptide sequencing; NMR could be used to study protein secondary structure.(C) CD data yields protein secondary structure; Mass spectroscopy could be used in peptide sequencing.(D) X-ray diffraction data yields interatomic distances; NMR yields electron density map.(E) Both CD and NMR could be used to study protein secondary structure.

30. Which of the following statements about protein-ligand binding is correct?(A) The Ka is equal to the concentration of ligand when all of the binding sites are occupied.(B) The Ka is independent of such conditions as salt concentration and pH.(C) The larger the Ka (association constant), the weaker the affinity.(D) The larger the Ka, the faster the binding.(E) The larger the Ka, the smaller the Ka (dissociation constant).

31. Which of the following statements about a plot of Vo vs. [S] for an enzyme that follows Michaelis-Menten kinetics is   false  ?(A) As [S] increases, the initial velocity of reaction Vo also increases.(B) At very high [S], the velocity curve becomes a horizontal line that intersects the y-axis at Km.(C) Km is the [S] at which V₁ = 1/2 max.(D) The shape of the curve is a hyperbola.(E) The y-axis is a rate term with units of mM/min.

32. DIFP inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme. This is an example of what kind of inhibition?(A) Non-competitive.(B) Competitive.(C) Irreversible.(D) Mixed.(E) pH.

33. Which one of the following statements is true?1. The substance that loses electrons is called oxidizing agent.2. There is entropy gain when ADP is phosphorylated.3. Flavin adenine dinucleotide (FAD) is a biological oxidizing agent.4. NADH, NADPH, FAD and coenzyme A all contain ADP unit.(A) 1 and 2 are true.(B) 3 and 4 are true.(C) 1 and 3 are true.(D) 2 and 4 are true.(E) All are true.

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