24. Which of the following is not used in the electrophoretic mobility shift assay (EMSA)?
(A) A radiolabeled DNA fragment
(B) A polyacrylamide gel
(C) A DNA binding protein
(D) DNase I

28. Which of the following is not used in the electrophoretic mobility shift assay (EMSA)? (A) a radiolabeled DNA fragment (B) a polyacrylamide gel (C) a DNA binding protein (D) DNase I

25. Which of the following is not a structural motif found in a DNA-binding domain? (A) Random-coil acidic domain (B) Zinc-finger (C) Helix-loop-helix (D) Homeodomain

26. Splice sites in pre-mRNA are marked by two universally conserved sequences contained (A) In the middle of the intron. (B) At the ends of the exons. (C) At the ends of the introns. (D) None of the above

27. Which of the following RNA functions in the removal of introns from pre-RNAs. (A) snRNA (small nuclear RNA) (B) snoRNA (small nucleolar RNA) (C) siRNA (small interfering RNA) (D) miRNA (micro RNA)

28. Indicate the order in which the following steps occur in the production of a mature mRNA. (A) Initiation of transcription, splicing, addition of 5' cap, addition of polyA. tail, transport to cytoplasm (B) Initiation of transcription, addition of 5' cap, splicing, transport to cytoplasm, addition of polyA. tail, (C) Initiation of transcription, addition of polyA. tail, addition of 5′ cap, splicing, transport to cytoplasm (D) Initiation of transcription, addition of 5' cap, addition of polyA. tail, splicing, transport to cytoplasm

29. Comparing mRNA molecules from human and Escherichia coli cells, which of the following is typically NOT true? (A) A human mRNA has a special 5' cap, while a bacterial mRNA does not. (B) A human mRNA has a poly-A tail, while a bacterial mRNA does not. (C) A human mRNA undergoes alternative splicing, while a bacterial mRNA does not. (D) A human mRNA contains noncoding sequences, while a bacterial mRNA does not. (E) A typical human mRNA encodes one protein, while many bacterial mRNAs encode several different proteins.

30. In the following schematic graph of a hypothetical set of RNA-seq data, the number of reads is plotted for a region of chromosome containing two genes, from samples obtained from two different tissues. Which gene (X or Y) do you think is more likely a "housekeeping" gene? Which region (1 or 2) within gene Y most likely corresponds to an exon?  (A) Gene X; region 1 (B) Gene X; region 2 (C) Both genes; region 1 (D) Gene Y; region 1 (E) Gene Y; region 2

31. Which of the following statements about splicing is correct? (A) All of the splicing require enzymatic activity provided by proteins (B) Splicing can occur during or after transcription (C) Splicing reactions occur only in cis between splice sites on the same molecular of RNA (D) Production of rRNA requires cutting and rejoining in separate reactions

32. Which of the following is not a feature of ribosomes? (A) Cytoplasmic and organellar ribosomes of eukaryotes are identical. (B) All ribosomes have one large subunit and one small subunit. (C) All ribosomes are composed of RNA and protein. (D) Ribosomes interact with both mRNA and tRNA.

33. The function of bacterial EF-Ts is to (A) Convert EF-Tu GDP to an active form. (B) Convert GTP to GDP. (C) Allow binding of aminoacyl-tRNAs to the A site of the ribosome. (D) Move the peptidyl-tRNA from the A site to the P site. (E) Deacylate tRNAs.

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