30. What amino acid catabolism needs α-keto acid decarboxylase? If the decarboxylase is defect, it will cause maple syrup urine disease.
(A) alanine
(B) valine
(C) tryptophan
(D) phenylalanine
(E) tyrosine.

31. According to the table above, which of the following DNA sequences is the putative coding region of the peptide? (A) ATG CCT TAC GAT GTG CCT GAT TAT GCT (B) TAA CCC TAT GAT GTA CCC GAC TAC GCC (C) TAT CCA TAA GAC GTC CCA GAT TAT GCA (D) TAC CCG TAT GAC GTC CCG GAC TAC GCG (E) TAG CCG TAC GAT GTT CCT GAT TAT GCC

32. To isolate the full-length gene, Wen-Liang will design synthetic DNA probes with 18 nucleotides to screen cDNA libraries prepared from viral infected serum. According to the table above, which of the following peptides will minimize the number of DNA probes for the screening? (A) YPYDVP (B) PYDVPD (C) YDVPDY (D) DVPDYA (E) All are the same

33. When he designs the PCR primer pairs that contain the restriction enzyme cutting sequences, which of the following primer pairs is his best choice? (A) the forward primer with BamH I; the reverse primer with Xho I (B) the forward primer with Xho I; the reverse primer with EcoR I (C) the forward primer with BamH I; the reverse primer with Hind III (D) the forward primer with EcoR I; the reverse primer with Xho I (E) the forward primer with Hind III; the reverse primer with BamH I

34. When Wen-Liang transfects the subcloned plasmid into the HEK 293T cell line and checks the gene expression by running the SDS-PAGE, what is the protein size he will expect to observe on the gel? (A) 293 ka (B) 64 ka (C) 1734 ka (D) 578 ka (E) 193 ka

35. To further confirm the gene expression, Wen-Liang can use the following methods EXCEPT (A) Southern blot (B) Northern blot (C) Western blot (D) Quantitative real-time PCR (E) None of them

36. In terms of chemical properties, which of the following amino acids is lysine most similar to? (A) Arginine (B) Phenylalanine (C) Serine (D) Aspartic acid (E) Proline

37. Chymotrypsin preferentially cleaves after amino acids tryptophan, tyrosine, and phenylalanine in a protein. To a lesser extent, it may also cleave after leucine, methionine, and histidine. Which of the following peptides is NOT a product of chymotrypsin? (A) RSIAF (B) NWPQALPSVL (C) QIAM (D) EPDTDW (E) SIAFSR

複選題38. SDS-PAGE is a method in which proteins coated with negatively charged detergent SDS are separated along a gel matrix in the presence of an electric field. The method separates proteins based on relative molecular weights of proteins. Which of the following statements about SDS-PAGE is TRUE? (A) Larger proteins bind more negatively charged SDS and run faster (B) Larger proteins run slower because they are heavier (C) Larger proteins run slower because they face a greater resistance (D) Proteins run from anode to cathode (E) Larger proteins separate better in a higher percentage SDS-PAGE gel

39. The figure on the right summarizes the effects of pH on oxygen binding affinity of hemoglobin (the Bohr effects). Based on the figure, which of the following statement is TRUE? (A) Curve A represents binding in the highest pH (B) Curve B represents binding in the highest pH (C) Curve C represents binding in the highest pH (D) Curve A has the highest affinity (E) Curve A bind the highest amount of oxygen for a given oxygen pressure

40. Which of the following statements about isoelectric point is TRUE? (A) An isoelectric point is the pH where a molecule has equal negative and positive charges (B) Proteins have isoelectric points but amino acids do not (C) Positive charges of a protein increase as pH increases (D) At the isoelectric point, a molecule moves to the anode in an electric field (E) Since proteins carry zero charges at isoelectric points, electrophoresis cannot separate different proteins based on isoelectric points

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