4. Which below method cannot be used to disrupt cells for protein purification?
(A)Cell lysis
(B)French press
(C)Enzyme digestion
(D)Grinding
(E)Centrifugation

5. Which of the following enzymes possesses both RNA-dependent DNA polymerase and DNA-dependent DNA polymerase activities? (A)DNA polymerase I (B)DNA polymerase II (C)DNA polymerase III (D)Klenow fragment (E)Reverse transcriptase

6. Which of the following polymerase synthesizes 5S rRNA? (A)RNA polymerase I (B)RNA polymerase II (C)RNA polymerase III (D)Telomerase (E)Reverse transcriptase

7. Which of the following enzymes is a ribozyme? (A)RNase A (B)RNase D (C)RNase H (D)RNase P (E)RNase III

8. Which of the following factors is critical for the maintenance of lysogenic state of lambda phage? (A)Cro protein (B)Lambda repressor (C)N protein (D)Q protein (E)Sigma factor

9. The expression of kappa light chain of antibodies is controlled by which of the following gene regulations? (A)Gene deletion (B)Gene translocation (C)Gene amplification (D)Gene conjugation (E)Gene insertion

10. Which of the following templates is NOT a substrate for polymerase chain reaction (PCR)? (A)Double strand DNA (B)Single strand DNA (C)Circular form of DNA (D)mRNA (E)Plasmid DNA.

11. At replication forks in E. coli: (A)DNA helicases make endonucleolytic cuts in DNA. (B)DNA primers are degraded by exonucleases. (C)DNA topoisomerases make endonucleolytic cuts in DNA. (D)RNA primers are removed by primase. (E)RNA primers are synthesized by primase.

12. The function of the eukaryotic DNA replication factor PCNA (proliferating cell nuclear antigen) is similar to that of the B-subunit of bacterial DNA polymerase III in that it: (A)facilitates replication of telomeres. (B)increase the processivity of replication. (C)has a 3' → 5' proofreading activity. (D)increases the speed but not the processivity of the replication complex. (E)participates in DNA repair.

13. Which of the following statements regarding plasmid cloning vectors is correct? (A)Circular plasmids do not require an origin of replication to be propagated in E. coli. (B)Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid. (C)Plasmids do not need to contain genes that confer resistance to antibiotics. (D)Plasmid vectors must carry promoters for inserted gene fragments. (E)The copy number of plasmids may vary from a few to several hundred.

14. In homologous recombination in E. coli, the protein that assembles into long, helical filaments that coat a region of DNA is: (A)RecA protein. (B)RecBCD enzyme. (C)DNA methylase. (D)DNA polymerase. (E)Histone.

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