47.Which of the following fat-soluble vitamins is involved in blood clotting?
(A) vitamin A
(B) vitamin D
(C) vitamin E
(D) vitamin K

48.Glycolysis is only partially reversible because of energy barriers at the reactions catalyzed by: (A) hexokinase, triose phosphate isomerase and pyruvate kinase (B) hexokinase, pyruvate dehydrogenase and phosphoenolpyruvate carboxykinase (C) hexokinase, phosphofructokinase, and pyruvate kinase (D) hexokinase, glyceraldehyde 3-phosphate dehydrogenase and pyruvate kinase

49.Which of the following is true about the difference between fatty acid degradation and biosynthesis? (A) Fatty acid degradation is in cytosol while its biosynthesis is in mitochondria (B) Fatty acid degradation starts at carboxyl end while its biosynthesis starts at methyl end (C) The final product for fatty acid degradation is propinyl CoA while the final product for its biosynthesis is acetyl CoA (D) The malonyl-CoA is involved in the fatty acid degradation, but the malonyl-CoA is not involved in its biosynthesis

50.Acetyl-CoA for de novo fatty acid biosynthesis is formed by the energy-dependent cleavage of: (A) pyruvate (B) oxaloacetate (C) citrate (D) α-ketoglutarate

51.The acyl donor for cholesterol ester formation in blood is: (A) phosphatidylcholine (B) palmitoyl-CoA (C) triglycerides (D) acyl carrier protein

52.Which of the following sequence is required for purification on the Nickel column? (A) Lys tag (B) His tag (C) Gly tag (D) Met tag

53.When making the antibody, which region of the synthetic peptides should be used? (A) Hydrocarbon region (B) Hydrophobic region (C) Hydrophilic region (D) Hydrolytic region

54.What is the major difference between genomic DNA and cDNA? (A) intron (B) exon (C) nucleotide (D) None of the above

55.The first dimensional separation in two-dimensional gel electrophoresis is to separate the molecule based on: (A) the size (B) the net charge (C) the structure (D) the affinity

56.Which of the following is not a polysaccharide? (A) Chitin (B) cellulose (C) pectin (D) lignin

57.Which reaction generates NADH in glycolysis? (A) glucose → glucose-6-phosphate (B) fructose-6-phosphate → fructose-1,6-bisphosphate (C) glyceraldehydes-3-phosphate → 1,3-bisphosphoglycerate (D) phosphoenolpyruvate → pyruvate

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