8. The cDNA is
(A) circular DNA;
(B) complementary DNA;
(C) central DNA;
(D) cloned DNA;
(E) cut DNA.

9. All of the following items are biological macromolecules except(A) Protein; (B) Carbohydrate; (C) Nucleic acid; (D) Fats; (E) Vitamin

10. Which of following descriptions is not correct for Mendel's laws of inheritance?(A) One allele can be dominant over the other, recessive, allele; (B) If each parentcarries two copies of a gene, the parents are diploid for that gene; (C) A gene can existin different forms called alleles; (D) An organism that has two identical alleles for agene is said to be homozygous for that gene; (E) Sex cells, or gametes, are haploid,containing two copies of each gene.

11. Which of the following techniques is most useful to amplify a specific DNA fragment in vitro? (A) Southern analysis; (B) DNA fingerprinting; (C) Polymerase chain reaction; (D) DNA sequencing; (E) RACE

12.β-galactosidase cleaves the disaccharide lactose into _______and_______.(A) glucose, galactose; (B) glucose, fructose; (C) galactose, fructose; (D) galactose, galactose. (E) glucose, glucose.

13. Which of the following statements is not true about the λ phage?(A) DNA circularization in E.coli by 12 base cos cohesive ends; (B) The product of clgene is a repressor, which is important for lysogeny; (C) The expression of cro genemust be stimulated during lysogeny; (D) cIII is a protector of cII; (E) N and Q genesmust be turn on during lytic phase.

14. Which of the following statements is not true about histone? (A) The most common histone modification is methylation; (B) Core histones contain H2A, H2B, H3, and H4; (C) The DNA winding almost twice (1 and 3/4) around the core histones; (D) Most of the histones are also well conserved from one organism to another; (E) Histones are usually basic and have pronounced positive charge at neutral pH.

15. Heterochromain:(A) chromatin that is active; (B) It is found at the telomeres; (C) chromatin that isextended; (D) accessible to RNA polymerase; (E) contain most of the genes.

16. The following domains which can play DNA binding activity? (A) zinc fingers; (B) prolin-rich regions; (C) acid regions; (D) coiled-coil domain; (E)PRR domains.

17. The correct order of chromatin folding is (1) nucleosome formation (2) 30 nM fiber formation (3) radial loop structure (A) 1, 2, 3; (B) 3, 2, 1; (C) 2, 1, 3; (D) 2, 3, 1; (E) 1, 3, 2.

18. The consensus sequence of splicing signal at the first two bases and the last two bases of pre-mRNA are (A) GG-AG; (B) GU-AG; (C) CU-CG; (D) GU-AU; (E)GA-CG.

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