46. You discover a bacteria species in a deep ocean sample that can turn olive oil into pure gold. The bacteria do not replicate well at sea level, and one night all of your "Golden" bacteria die. What can you do to possibly retrieve the gold-making gene from the dead Golden bacteria?
(A) Transform a live bacterial strain with the Golden bacteria's DNA.
(B) Transduce a live bacterial strain with the Golden bacteria's DNA.
(C) Conjugate a live bacterial strain with the Golden bacteria.
(D) Transduce the Golden bacteria and then infect a live strain of bacteria with the transducing virus.

47. Pathogenic microorganisms and their hosts live in a close association with one another known as: (A) sociality (B) mutualism (C) parasitism (D) commensalism

48. You are studying the bacteria in the water of a nearby pond. From a 100 mL sample, you use a 0.5 mL subsample to inoculate a plastic dish filled with a semisolid nutrient medium that fosters growth of coliform bacteria. After an appropriate incubation period, you count 100 bacterial colonies growing on the surface of the medium. What conclusion can you make about the coliform bacteria in the pond water? (A) There is approximately one coliform bacterial cell per milliliter. (B) There are approximately 200 coliform bacterial cells per milliliter. (C) There is approximately one coliform bacterial species per milliliter. (D) There are approximately 200 coliform bacterial species per milliliter.

49. Consider a large algal colony like Cladophora or Microcystis, which plays host to a complex biofilm of bacteria. What is a specific benefit the algal host may receive from the relationship? (A) Oxygen (B) Vitamin B12 (C) A physical habitat (D) Glucose

50. An individual human can be thought of as a ________, consisting of a host and a variety of microbes. DNA from saliva or fecal samples, for example, would reveal the DNA genomes of both the host and its symbiotic microbes, known together as the ________. (A) holobiont, hologenome (B) hologenome, holobiont (C) microbiome, microgenome (D) microbiome, hologenome

51. You are observing the embryonic development of a new species in your laboratory. This species has indeterminate development. You note a mutation in which one of the blastomeres (early cells) mutates and loses its function. What do you hypothesize would happen to this being? (A) Nothing—the remaining blastomeres would accommodate the formation of the necessary structures of the embryo. (B) Immediate embryo death—the remaining blastomeres could not accommodate the formation of the necessary structures of the embryo. (C) Uncertain future for the embryo—depending on which blastomere was lost, the remaining blastomeres might accommodate the formation of the necessary structures of the embryo. (D) The embryo would certainly lack some features as a result of the lost blastomere.

52. Molecular-based phylogies place the phylum Platyhelminthes in the Protostomia. How do scientists best explain the morphological differences between flatworms and the other members of the Lophotrochozoa? (A) Modern Platyhelminthes do not have a coelom but did originate from an ancestor that had one. (B) The Platyhelminthes clade separated from other clades before coeloms evolved and consequently Platyhelminthes have never had a coelom. (C) Platyhelminthes originated from ancestors with a coelom and have retained it to the present time. (D) Platyhelminthes evolved from an ancestor without a coelom but developed one during the course of their evolution.

53. A frog loses its ability to undergo buccal pumping. What must happen to compensate for this loss? (A) The pumping of blood from the atria to the ventricle must increase. (B) The exchange of gases through the skin must increase. (C) The pumping of blood from the ventricle to the body must increase. (D) The diffusion of wastes through the kidneys must increase.

54. Metamorphosis is (A) changing of one body form to another within a species, such as the change from an aquatic tadpole to a terrestrial frog. (B) an intermediate condition, such as length of legs in mice between longer legs of some mice and shorter legs in others, a condition caused by Hox genes. (C) the developmental changing of a scale to a feather. (D) the evolutionary transition from fishes to amphibians.

55. Which of the following would NOT be an example of negative feedback control in homeostasis? (A) Blood volume and pressure drop due to minor bleeding. This decreased pressure causes the kidneys to increase water reabsorption to restore blood volume and pressure. (B) Exposure to cold water causes body temperature to drop. Decreased stimulation of heat receptors causes constriction of blood vessels near the skin and better retention of heat. (C) Glucose levels rise following consumption of a big dessert, triggering release of insulin which causes glucose levels to fall. (D) During the birth process, stretch receptors in the uterus stimulate the release of oxytocin from the pituitary gland. This hormone stimulates uterine muscle contraction that increases stimulation of the stretch receptors.

56. Sucralose is a non-caloric artificial sweetener. Its structure is similar to sucrose, but with three of sucrose's hydroxyl groups replaced by chloride. How do you predict sucralose behaves in the body? (A) Sucralose enters cells by diffusion. (B) Sucralose is unable to bind conventional sugar transport mediators and is excreted. (C) Sucralose binds to glucose transport mediators and is metabolized by normal metabolic pathways. (D) Sucralose does not enter the body.

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