5. Which of the following enzymes plays a direct role in the biosynthesis of collagen?
(A) Prolyl hydroxylase
(B) Tyrosine hydroxylase
(C) Choline oxidase
(D) Monoamine oxidase
(E) Tryptophan oxygenase

6. Some pharmaceutical companies try frantically to find inhibitors of telomerase. A telomerase inhibitor could, in theory, be used in an attempt to (A) Boost the synthesis of muscle proteins. (B) Prevent viral infections. (C) Cure cancer. (D) Cure AIDS. (E) Make people immortal.

7. Cyanide is a potent inhibitor of cell respiration that prevents the oxidation of all nutrients. Cyanide will therefore definitely reduce the cellular concentration of : (A) Heme groups. (B) FADH2. (C) CoA. (D) ATP. (E) SAM.

8. A hydropathy plot is used to: (A) Determine the water-solubility of a protein (B) Deduce the water content of a native protein (C) Estimate for the true molecular weight of a membrane protein (D) Deduce the quaternary structure of a membrane protein (E) Predict whether a given protein sequence contains membrane-spanning segments

複選題9. B-sheets are stabilized by which of the force? (A) Hydrophobic interaction (B) Vanderwall force (C) Hydrogen bonds between the (CO) and the (NH) units in the peptide backbone (D) Hydrogen bonding between the R groups of polypeptide chains (E) Hydrogen bonds between adjacent segments.

10. Sickle cell anemia is a molecular disease of hemoglobulin. The altered properties of hemoglobin S result from a single amino acid mutation. The following statement which is right? (A) a Leu instead of a Asp residue (B) a Ile instead of a Asn residue (C) a Val instead of a Glu residue (D) a Leu instead of a Gln residue (E) a Met instead of a Asn residue

11. When saturated, it was found that a solution containing 0.0001 M of enzyme A can catalyze the breakdown of 0.05 M substrate in 1 second. The kcat of enzyme A is: (A) (B) (C) (D) (E)

12. Hexokinase has a Km of 0.05 mM for D-glucose and a Km of 1.5 mM for D-fructose. Please choose the correct statement(s) based on this information. I: Hexokinase has a greater affinity for D-fructose than for D-glucose. II: D-fructose is a better substrate for hexokinase than D-glucose.  III: It takes less D-glucose for the reaction to reach "half " than D-fructose. IV: Hexokinase has a higher kcat for D-glucose than for D-fructose. (A) II (B)III (C)II, III, IV (D) I, II, III (E)II, III

13. According to the steady state kinetics, the initial velocity of an enzyme-catalyzed reaction at very low [S] can be represented as (A)(B) (C)(D) (E)

14. To express a human gene in E. coli, the expression vector must possess which of the following features? I. Promoter and terminater sequences for transcription.  II. Appropriate fusion tags. III. CEN and TEL sequences.  IV. Ribosome binding site. (A) I, II, III, IV (B) I, III, IV (C) II, III, IV (D) I, III (E) I, IV

15. Which of the following enzymes is absolutely required for the construction of cDNA library? (A) reverse transcriptase (B) polynucleotide kinase (C) terminal transferase (D) DNA polymerase I (E) DNA topoisomerase

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