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業餘無線電◆一等◆電磁相容性技術

業餘無線電◆一等◆射頻干擾的預防與排除

業餘無線電◆三等◆無線電規章與相關法規

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25. Which of the following statement is correct related to general and gene-specific transcription factors in eukaryotes? (A) TATA-box-binding protein (TBP) binds to the minor groove of DNA, bends the consensus TATA DNA, and is required for most members of class I, II and III genes. (B) For RNAP III, TBP is a component of the general transcription factor TFIIIC. (C) The bZIP transcription factors dimerize through a basic domain and bind to target DNA through a leucine zipper. (D) TFIIE and TFIIH are essential for promoter clearance and transcription elongation. (E) The Cys2His2-zinc finger of TFIIIA is composed of an antiparallel beta-sheet followed by an alpha-helix, and the beta-sheet recognizes and binds to specific DNA sequences in the major groove.

24. Which of the following statement is correct in eukaryotic transcription? (A) Yeast Gcn4 controls amino acid metabolism, containing a domain using the basic region to bind to DNA and helix-loop-helix to form a protein dimer. (B) The carboxyl-terminal domain of RNA polymerase is phosphorylated prior to the formation of the initiation complex. (C) By adding of excess acetyltransferase to in vitro transcription assay, it is likely lead to a tighter association of histone with DNA, resulting in reduced transcription. (D) TAF1 of the general transcription factor TFIID is a helicase and a histone acetyltransferase. (E) The high mobility group protein (HMG I(Y)) plays a role in interferon-ẞ (IFN-ẞ) transcription by bending the DNA, causing more activators to bind and interact with each other to enhance transcription.

23. Which of the following is true for eukaryotic Class I and III general transcription factors, except: (A) TFIIIB and TFIIIC are required for all "classical" class III genes. (B) TFIIIC is an assembly factor that helps TFIIIB to bind to a region just upstream of the transcription start site. (C) Human SL1 is an assembly factor that helps the UPE-binding factor of RNA polymerase I to bind to the core promoter element. (D) TFIIIA is a zinc finger protein & is required for transcription of the 5S rRNA genes, but not the tRNA genes. (E) For the class I transcription, human SL1 consists of TATA-binding protein (TBP) and TBP-associated factors (TAFs), which are different from TAFs found in TFIID.

10. Why is a frameshift mutation located at the beginning of a coding region generally more detrimental to protein function than a missense mutation? Briefly explain the reason. (4 pts)

9. You observe that adding DNase to a bacterial culture prevents the bacteria from acquiring a new hereditary trait. Based on this observation, which gene transfer mechanism is occurring (Transformation, Transduction, or Conjugation)? Explain your reasoning. (4 pts)

8. In quantitative PCR (qPCR), why do we focus on the Ct value (Threshold Cycle) rather than the final amount of product at the end of the reaction? (3 pts) Briefly define what the Ct value represents. (3 pts)

高中英文

講師：【站僕】摩檸Morning.

簡介：高中英文

高中數學

講師：【站僕】摩檸Morning.

簡介：高中數學

高中生物

講師：【站僕】摩檸Morning.

簡介：高中生物

小法點

描述：封面

七下生物3-3

描述：字醜非常抱歉orz

信託業務人員

描述：筆記

49.依據「職業安全衛生管理辦法」之規定，雇主對高壓氣體特定設備、高壓氣體容器及第一種壓力容 器應每月定期實施檢查一次，下列規定何者錯誤？ (A)本體有無損傷、變形(B)壓力表及溫度計及其他安全裝置有無損傷 (C)平台支架有無嚴重腐蝕(D)配線、配電盤、開關及控制裝置有無異常

20.考試是學生主要的壓力來源之一，面對即將進入國三的學生，國中基本能力測驗是一場不可避免的重大考 試。某國中校長希望在開學之初找出對壓力反應傾向較高的學生，以便協助因應考試壓力。下列測驗何者最 合適？ (A)情境焦慮量表 (B)特質焦慮量表 (C)貝克憂鬱量表(BDI-II) (D)貝克焦慮量表(BAI)

34.本月因工作量增加，員工有加班，並請了2名臨時工讀生，下列對成本之敘述，何者正確？ (A)員工基本薪水＋加班費屬半變動成本 (B)臨時工讀生屬於半變動成本 (C)臨時工讀生屬於固定成本 (D)員工基本薪水屬變動成本

20.甲公司於×5 年 1 月 1 日有保留盈餘$500,000，流通在外普通股股數為 200,000 股，面額$10。當年度公司發生之交易如下：1.宣告現金股利每股$1；2.宣告並發放 5%的股票股利，目前股票市價為每股$18；3.當年度淨利$460,000。甲公司×5 年 12 月 31 日保留盈餘餘額為多少？ (A) $760,000 (B) $660,000 (C) $580,000 (D) $960,000

5.流式細胞儀操作中，下列敘述何者錯誤？ (A)與 488 nm 雷射光垂直的光徑偵測器無法提供細胞大小的數據 (B)激發的紅色螢光與綠色螢光信號可能會互相干擾  (C)細胞沒有染色則無法看出細胞內的顆粒密度 (D)細胞表面分子可以活染不需固定細胞

7.下列細胞激素何者無法幫助肥大細胞的發育與成熟？ (A)IFN-γ (B)IL-3 (C)IL-4 (D)IL-9