42. Which of the following compounds could served as the precursor of a ligand that involved in ion flux?
(A) lysine
(B) threonine
(C) glycerol
(D) inositol
(E) dolichol

43. Please select one suitable probe for exploring sodium channels in the membrane. (A) actinomycin (B) puromycin (C) bungarotoxin (D) cholera toxin (E) saxitoxin

44. Which of the following assays can be used to determine protein-protein interaction in cell? (A) polymerase chain reaction (B) scatchard analysis (C) polyacrylamide gel electrophoresis (D) radioimmunoassay (E) fluorescence resonance energy transfer

45. Which of the following polysaccharides has the highest density of negative charge in one molecule at pH 7? (A) keratin (B) heparin (C) chitosan (D) hyaluronate (E) chondroitin 

46. Please describe what type of polymer of this gel? (A)SDS-PAGE (B) Reduced SDS-PAGE (C) Agarose gel (D)All of above (E) None of above

47. What is the molecular weight of the expression vector (A)~4.7 kb (B) 5.4 kb (C)~6.2 kb (D)~6.8 kb (E) ~ 8.0 kb

48. What is the molecular weight of the cDNA insert (A)~3.0 kb (B)~4.0 kb (C)~400bp (D) ~600bp (E)~1.5kb

49. How many repeated inserts in this construct: (A) 0 (B) 1 (C) 2 (D) 3 (E) 4

50. Which glycosaminoglycan are the highest level found in mast cells? (A) heparan sulfate (B) heparin sulfate (C) chondroitin sulfate (D) keratin sulfate (E) hyaluronic acid

1. Which of the following statements about the EGF and LCGF treatments is LEAST likely? (A) Some cell types have receptors for more than one growth factor. (B) Hepatoma cells have an LCGF receptor but not an EGF receptor. (C) EGF and LCGF trigger growth by binding with different affinities to the same receptor. (D) Growth factors show some specificity regarding target cell type. (E) Both normal and tumor cells can respond to growth factors.

2. Cultured fibroblasts were labeled with 32P-ortho-phosphate. Subsequent EGF treatment increased the radioactivity detected in a small subset of total cell proteins. Which of the (A) EGF acts as a protein phosphatase. (B) EGF activates a protein kinase. (C) EGF activates an ATPase. (D) EGF is phosphorylated. (E) The activated receptor acts as a protein phosphatase.

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