45. Which of the following polysaccharides has the highest density of negative charge in one molecule at pH 7?
(A) keratin
(B) heparin
(C) chitosan
(D) hyaluronate
(E) chondroitin 

46. Please describe what type of polymer of this gel? (A)SDS-PAGE (B) Reduced SDS-PAGE (C) Agarose gel (D)All of above (E) None of above

47. What is the molecular weight of the expression vector (A)~4.7 kb (B) 5.4 kb (C)~6.2 kb (D)~6.8 kb (E) ~ 8.0 kb

48. What is the molecular weight of the cDNA insert (A)~3.0 kb (B)~4.0 kb (C)~400bp (D) ~600bp (E)~1.5kb

49. How many repeated inserts in this construct: (A) 0 (B) 1 (C) 2 (D) 3 (E) 4

50. Which glycosaminoglycan are the highest level found in mast cells? (A) heparan sulfate (B) heparin sulfate (C) chondroitin sulfate (D) keratin sulfate (E) hyaluronic acid

1. Which of the following statements about the EGF and LCGF treatments is LEAST likely? (A) Some cell types have receptors for more than one growth factor. (B) Hepatoma cells have an LCGF receptor but not an EGF receptor. (C) EGF and LCGF trigger growth by binding with different affinities to the same receptor. (D) Growth factors show some specificity regarding target cell type. (E) Both normal and tumor cells can respond to growth factors.

2. Cultured fibroblasts were labeled with 32P-ortho-phosphate. Subsequent EGF treatment increased the radioactivity detected in a small subset of total cell proteins. Which of the (A) EGF acts as a protein phosphatase. (B) EGF activates a protein kinase. (C) EGF activates an ATPase. (D) EGF is phosphorylated. (E) The activated receptor acts as a protein phosphatase.

3. When the divalent antibody was made monovalent by separating the antigen-binding domains (Fabs) from the constant domain (F), the Fab portion, but not the F. portion, bound the EGF receptor. Neither portion triggered cell proliferation. Which of the following conclusions is best supported by these results? (A) EGF binds a divalent antibody but not a monovalent antibody. (B) Signal transduction requires EGF or a monovalent antibody. (C) Binding of the Fab portion alone leads to internalization of the receptor. (D) Only a divalent antibody recognizes the binding site for EGF. (E) Dimerization of EGF receptors by a divalent antibody leads to signal transduction.

4. Which of the following accounts for the extent of proliferation observed in the retrovirus-infected cells not treated with growth factors? (A) The v-erbB product has a function similar to that of activated EGF receptor. (B) EGF binds to both the EGF receptor and the v-erbB product. (C) The v-erbB product activates both the LCGF and the EGF receptors. (D) The v-erbB product antagonizes the action of the EGF receptor. (E) The v-erbB product induces secretion of EGF and LCGF.

5. Which of the following enzymes plays a direct role in the biosynthesis of collagen? (A) Prolyl hydroxylase (B) Tyrosine hydroxylase (C) Choline oxidase (D) Monoamine oxidase (E) Tryptophan oxygenase

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